Dear Visitor, this website is dedicated to my results done in cooperation with various international projects. In my research I use interdisciplinary approaches for solving of biological problems.
The most common techniques include sequence alignment, molecular modeling, molecular dynamics and docking of biological macromolecules.
I have completed my PhD in Molecular Biology at the Protein Engineering and Bioinformatics Department, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine (https://imbg.org.ua/en/).
Via oral and poster presentations I participated in more than 25 International Conferences, Congresses and Workshops in EU countries, supported by personal Travel Grants and Certificates from FEBS/IUBMB/EMBO, EBSA, iNEXT (HORIZON 2020), ENABLE (Biotechnology Business Institute), EGI-Engage (HORIZON 2020), PRACE/Cineca, HPC-Europa3 (Horizon 2020), eSSENCE, COMBIOM (7th EU Framework Program) and WIPO, among others. In addition to numerous Awards for the best presentations, I was awarded a four-year Fellowship as one of the best PhD students at the National Academy of Sciences of Ukraine (2012-2014, 2016-2018), Fellowship of President of Ukraine (2018-2019), Kyiv City Mayor Prize (2018), Shevchenko Scientific Society, Inc Award (New York, USA, 2018) and State Prize of President of Ukraine for young scientists in 2018.
My dissertation combines molecular modeling, molecular docking, MD simulations and analysis of conformational changes in human tyrosyl-tRNA synthetase (HsTyrRS) and its mutant forms, associated with neurodegenerative diseases like Charcot-Marie-Tooth neuropathy (degeneration of peripheral nerve fibers). All automation of HPC computations was supported by in-house developed MolDynGrid Virtual Laboratory (https://moldyngrid.org) as part of the Ukrainian National Grid (https://ung.in.ua/) and European Grid Infrastructure (https://www.egi.eu/). Our research supports the idea that the lack of IL8-like (Interleukin 8) cytokine activity in the full-length HsTyrRS is explained by interactions between N- and C-terminal modules, which block the ELR motif [Savytskyi et al, 2013, J Mol Recognit, 113-120]. We found the new role of connective peptide 1 (CP1) in mammalian tyrosyl-tRNA synthetase. Formation of strong interaction between Lys154 (located in CP1) and γ-phosphate suggests the additional role of CP1 insertion as an important factor for ATP binding. [Kravchuk, Savytskyi et al., 2017, J. Biomol. Struct. Dyn., 2772–2788]. Novel β-sheet formation was observed in the K147-E157 region in G41R and 153-156delVKQV mutant forms of HsTyrRS in the CP1 region of the Rossmann fold. The results support the idea, that defects of the intermolecular interfaces of complexes of mutational forms of HsTyrRS with cognate tRNA(Tyr) and/or translation elongation factor eEF1A may be a common molecular mechanism of Charcot-Marie-Tooth disorder.
I have authored more than 40 publications (11 papers in international journals with impact factor up to 3.5). I have participated in 7 local and 4 international research projects related to Computational Structural Biology, High-Performance Computing and Grid-infrastructures. As a Research Group Leader, I have developed strong and productive collaborations with students in molecular dynamics of mammalian TyrRS in complex with tRNA and ligands, High-Performance Computing, etc. It resulted in the graduate studies of Vladyslav Kravchuk at the Institute of Science and Technology (IST) Austria and Master’s study of Valentyn Petrychenko at Georg-August-Universität Göttingen, Germany.
You can find my complete CV at https://alstudio.org/savytskyi/about-oleksandr/cv/ in more detail.
Oleksandr V. Savytskyi. (updated 17.02.2022)